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Guidelines for sampling for Virus Indexing

Guidelines for sampling of tissue culture plants

  • In case of mother culture every mother plant (clone) will be sampled for virus testing prior to initiation into tissue culture production.
  • In case of tissue culture raised plants (ex-agar plants / hardened plants), the following scale of sampling for virus and or/quality (genetic fidelity) testing, as the case may be, depending on the lot (batch) size of tissue culture plants produced, just prior to dispatch / shipment of consignment.
  • Lot size: Number of tissue culture plants to be sampled
    • Up to 1000 Nos 1% plants subject to a minimum of 10 Nos
    • 1001 to 10000 Nos 0.5% of plants subject to a minimum of 10 Nos
    • 10001 to 100000 Nos 0.1% of plants subject to a minimum of 50 Nos
  • Finally a random sample of 10 plants will be collected out of the composite sample drawn from each batch of tissue culture production separately for virus / quality testing without contamination.
  • In case of sampling for quality (genetic fidelity) testing, sample from mother plant (clone) from which tissue culture plants produced will also be collected and forwarded for testing.
  • Packing/ Labelling/ Forwardal of samples for testing: The sample collected will be blotted dry to remove excess moisture before packing. The sample will be placed in between paper towels, packed in self sealing / zip-lock polythene bags of appropriate size. The sample will be affixed with a label and kept in a ventilated card board box and /or thermocool box for forwardal to Sugarcane Breeding Institute, Coimbatore within specified time under cool conditions.
  • The packing box will be marked on top of the box with appropriate instructions such as “Handle with care/Tissue Culture Plants/ Rush Delivery” and either couriered or delivered in person to the Plant Pathology Laboratory within 24 hrs period under cool conditions.


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