Sugarcane Breeding Institute has been designated as a National DUS (Distinctness, Uniformity and Stability) centre for Sugarcane by the Protection of Plant Varieties & Farmers' Rights Authority (PVP). The institute has three centres for DUS testing of sugarcane varieties. The facilities for DUS testing were developed at Coimbatore and Agali (SBI Research Centre, Agali) for tropical sugarcane varieties and at Karnal (SBI Regional Centre, Karnal) for sub-tropical varieties.

The reference and example varieties of sugarcane for DUS testing are being maintained and characterized at these centres. National Test Guidelines for DUS Testing of sugarcane have also been developed and notified by this Institute.